Microsoft word - cv soumya 2013 new

SOUMYANANDA CHAKRABORTI
Department of Signaling Neurobiology and Cancer, Institut Curie
Bat 110 - Centre Universitaire, 91405 Orsay Cedex, France
Cell: 919432068202, email: [email protected]
Date of Birth: 30th October, 1983
Present Position: Postdoctoral researcher at Institut Curie, France (Dept of Signaling,
Neurobiology and Cancer). Mentor: Carsten Janke.
Research Topic: Impact of tubulin heterogeneity on biological functions and biophysical
properties of microtubules

Education

2008-2013
Ph.D studentship at Bose Institute (University of Calcutta) India
Thesis Title: The effect of ZnO nanoparticles on proteins and cells, and the
recognition of curcumin by tubulin.
Academic Supervisor: Prof. Pinak Chakrabarti
MSc in Biochemistry from the University of Calcutta 2001-2004 BSc (Hons) in Chemistry from the University of Calcutta

PUBLICATIONS: (Cumulative Impact factor = 57)
1. Stable and potent analogs derived from the modifications of the dicarbonyl moiety of
curcumin. Chakraborti S.; Dhar G.; Dwivedi V.; Das A.; Poddar A.; Chakraborti G.; Basu
G.; Chakrabarti P.; Surolia A.; Bhattacharyya B. Biochemistry, 2013, 52, 7449-7460.
2. Green synthesis of silver nanoparticles using glucan 1 from mushroom and study of
antibacterial activity. Sen, IK.; Mandal, AK.; Chakraborti, S.; Dey, B.; Chakraborty, R.;
Islam, SS. International Journal of Biological Macromolecule, 2013, 62, 439-449.
3. The effect of the binding of ZnO nanoparticle on the structure and stability of α-Lactalbumin: a
comparative Study. Chakraborti, S*.; Sarwar S .; Chakrabarti, P*. Journal of Physical
chemistry B, 2013, 117(43), 13397-13408. (*author for Correspondence).
4. Molecular basis of inactivation of metronidazole-resistant Helicobacter pylori using
polyethyleneimine functionalized ZnO nanoparticles. Chakraborti, S.; Bhattacharya S.;
Chowdhury,
doi:10.1371/journal.pone.0070776. (Times Cited 1)
5. Partial characterization and flocculating behavior 1 of an exopolysaccharide produced in
nutrient poor medium by a facultative oligotroph Klebsiella sp. PB12. Mandal, A.K.;
Yadav, K.K.; Sen, I.K.; Kumar, A.; Chakraborti, S.; Islam, S.S.; Chakraborty, R. Journal
of Bioscience and Bioengineering, 2013, 115, 76-81. (Times Cited 2)
6. Discrimination of ligands of different flexibility resulting from the plasticity of the
binding site in tubulin. Chakraborti, S.; Chakravarty, D.; Gupta, S.; Chatterjee, B.P.;
Dhar, G.; Poddar, A.; Panda, D.; Chakrabarti, P.; Dastidar, S.G.; Bhattacharyya, B.
Biochemistry, 2012, 51(36), 7138-7148. (Times Cited 2)
7. Flocculating property of extracellular polymeric substances produced by a biofilm-
forming bacterium Acinetobacter junii BB1A. Yadav, K.K.; Mandal, A.K.; Sen, I.K.;
Chakraborti, S.; Islam, S.S.; Chakraborty, R. Appl. Biochem. Biotechnol. 2012, 168,
1621-1634. (Times Cited 4)

8. Interaction of Polyethyleneimine Functionalized ZnO Nanoparticles with Bovine Serum
Albumin. Chakraborti, S.; Joshi, P.; Chakravarty, D.; Shanker, V.; Ansari, Z.A.; Singh,
S.P.; Chakrabarti, P. Langmuir, 2012, 28(30), 11142-11152. (Times Cited 9)

9. CIL-102 binds to tubulin at colchicine binding site and triggers apoptosis in MCF-7 cells
by inducing monopolar and multinucleated cells. Gireesh, K.K.; Rashid, A.; Chakraborti,
S.; Panda, D.; Manna, T. Biochem. Pharmacol. 2012, 84(5), 633-645.

10. Anticancer activity of chloroquine-gold nanoparticles against MCF-7 breast cancer
cells. Joshi, P.;* Chakraborty, S.;* Shanker, V.; Ansari, Z.A.; Singh, S.P.; Chakrabarti, P.
Colloids Surf. B. 2012, 95,195-200 (*Contributed equally). (Times Cited 7)

11. ZnO nanoparticles as an antibacterial agent against E.coli. Joshi, P.; Chakraborti, S.;
Chakrabarti, P.; Singh, S.P.; Ansari, Z.A.; Husain, M.; Shanker, V. Sci. adv. Mater. 2012,
4, 173-178. (Times Cited 9)

12. Curcumin recognizes a unique binding site of tubulin. Chakraborti, S.; Das, L.;
Kapoor, N.; Das, A.; Dwivedi, V.; Poddar, A.; Chakraborti, G.; Janik, M.; Basu, G.; Panda,
D.; Chakrabarti, P.; Surolia, A.; Bhattacharyya, B. J. Med. Chem. 2011, 54 (18), 6183-
6196. (Times Cited 18)
13. A multiple antibiotic and serum resistant oligotrophic strain, Klebsiella pneumoniae
MB45 having novel dfrA30, is sensitive to ZnO QDs. Kumar, A.; Chakraborti, S.; Joshi,
P.; Chakrabarti, P.; Chakraborty, R. Ann. Clin. Microbiol. Antimicrob. 2011, 10, 19.
(Times Cited 3)
14. Contrasting effect of gold nanoparticles and nanorods with different surface
modifications on the structure and activity of bovine serum albumin. Chakraborty, S.;
Joshi, P.; Shanker, V.; Ansari, Z.A.; Singh, S.P.; Chakrabarti, P. Langmuir, 2011, 27 (12),
7722-7731. (Times Cited 31)
15. Binding of chloroquine-conjugated gold nanoparticles with bovine serum albumin.
Joshi, P*.; Chakraborty, S*.; Dey, S.; Shanker, V.; Ansari, Z.A.; Singh, S.P.; Chakrabarti,
P. J. Colloid Interface Sci. 2011, 2 (355), 402-409.(*Contributed equally) (Highlighted as
Article of this month (February) by colloid and Nanomedidine 2012). (Times Cited 20)
16. The effect of zinc oxide nanoparticles on the structure of the periplasmic domain of the
Vibrio cholerae ToxR protein. Chatterjee, T.; Chakraborti, S.; Joshi, P.; Gupta, V.; Singh,
S.P.; Chakrabarti, P.; FEBS J. 2010, 277 (20), 4184-4194. (Times Cited 9)
17. Structure and activity of lysozyme on binding to ZnO nanoparticles. Chakraborti, S.;
Chatterjee, T.; Joshi, P.; Poddar, A.; Bhattacharyya, B.; Gupta, V Singh, S.P.; Chakrabarti
P. Langmuir, 2010, 26 (5), 3506-3513. (Times Cited 41)

18. Role of surface adsorbed anionic species in antibacterial activity of ZnO quantum dots
against Escherichia coli. Joshi, P.; Chakraborti, S.; Chakrbarti, P.; Haranath, D.; Shanker,
V.; Ansari, Z.A; Singh, S.P.; Gupta, V. J. Nanosci. Nanotechnol. 2009, 9(11), 6427-6433.
(Highlighted in Nature India, DOI:10.1038/nindia.2009.339; Published online 24
November 2009) (Times Cited 25)
Manuscript under revision/submission/preparation
1. The mechanism of action of PEG functionalized Zinc Oxide against different breast
cancer cell lines. Chakraborti, S.; Adhikary, A.; Saha, S.; Das, T.; Chakrabarti, P.
2. Bactericidal effect of polyethyleneimine capped ZnO nanoparticles on multiple antibiotic
resistant bacteria harboring genes of high pathogenicity island. Chakraborti, S.; Mandal,
A.K.; Sarwar, S.; Singh, P.; Chakraborty, R.; Chakrabarti, P.
PATENTS
“A novel surface-functionalized ZnO-nanoparticles exhibiting therapeutics properties, and
process for preparing the same”; Chakraborti, S.; Joshi, P.; Das, T.; Chakrabarti, P.;
Chakraborty, R.; Mandal, A.K. (Indian: 1032/KOL/2012 dated 23.09.2012)

ORAL PRESENTATIONS / WORKSHOP / CONFERENCE POSTER
Attended International Conference on Yeast Genetics and Molecular Biology at Frankfurt
University, Germany, during Aug 29 – Sept 3, 2013.

National Symposium on Micro and Macro Resources in Biomolecular Technology,
organized by Department of Biotechnology, North Bengal University, held at Siliguri,
during February 25-26, 2013. (Best Oral Presentation Award)

International Conference on Biomolecular Forms and Functions organized by Molecular
Biophysics Unit, Indian Institute of Science, held at Bangalore during January 8-11, 2013.

International Interdisciplinary Science Conference (I-ISC) 2012 on “Protein Folding and
Diseases” organized by Center for Interdisciplinary research in Basic Sciences, Jamia
Millia Islamia University, held at New Delhi during December 8-10, 2012.
National Fluorescence Workshop (FCS 2012) organized by Fluorescence Society of India
held at Kolkata, India during December 3-7, 2012.

“Colloid and Nanomedicine 2012”, organized by Elsevier held at Amsterdam, Netherland
during July 15th-17th, 2012.

2nd International Conference on “Perspective of Cell Signaling and Molecular Medicine”,
organized by Bose Institute held at Bose Institute, Kolkata, during January 8th -11th, 2012.
(Best poster award)

“Conference on Informatics & Integrative Biology (CIIB-2011)”, organized by: Centre for
Bioinformatics, Bose Institute held at Bose institute, Kolkata, during December 14th – 16th,
2011. (Best poster award)
7th Asian Biophysics Association (ABA) symposium and annual meeting of the Indian
Biophysical Society (IBS), organized by: Indian Biophysical Society held at Indian Habitat
Centre, New Delhi, during January 30th -2nd February, 2011
National Symposium on “Cellular and molecular Biophysics”, organized by Indian
Biophysical Society held at Center for Cellular and Molecular Biology (CCMB),
Hyderabad, during January 22-24, 2009.
EMBO world lecture course on “Recent Development in Macromolecular Crystallography”
held at National Chemical Laboratory (NCL), Pune, during 9 -14th November, 2008. Jointly
organized by NCL, Pune and European Molecular Biology Laboratory, Hamburg,
Germany,

International symposium on “Complex Disease: Approaches to gene identification and
Therapeutic Management” held at Saha Institute of Nuclear Physics (SINP), Kolkata,
during 25-26th September, 2008.

Joint bilateral seminar “Antimicrobial Drug Resistance and the Development of New
Application” held at Indian National Science Academy (INSA) during 22-25th November,
2007. Jointly organized by INSA & German Academy of Science, Leopoldina.
International symposium “NANO-BIO INTERFACE 2006” held at SINP, Kolkata during
1-3rd March, 2006. Organized by Dr B.C.Guha Center for Genetic Engineering &
Biotechnology, University of Calcutta.
International conference of “Free Radical and Antioxidant in Health Disease and
Radiation”, organized by Biochemistry research wing, Institute of Post Graduate Medical
Education & Research, India.

International symposium on “Teaching, Research & Exploration in Biochemistry: 50 years
of journey” during January 6-7, 2006. Organized by: golden jubilee celebration committee,
Department of Biochemistry University of Calcutta at Ballygung Science College, Calcutta,
India.

TECHNICAL SKILLS
 Synthesis and characterization of nanoparticles (scanning electron microscopy,
transmission electron microscopy, energy dispersive x-ray, powder x-ray diffraction,
Fourier transformed infrared spectroscopy, atomic force microscopy, atomic absorption
spectroscopy).

 Standard molecular biology techniques, protein expression and purification involving
(gel filtration, ion exchange and affinity chromatography HPLC, FPLC systems and real
time PCR).
 Biophysical techniques for protein characterization (UV-VIS spectroscopy, fluorescence
spectroscopy, circular dichroism, isothermal caloriemetry, dynamic light scattering, surface
plasmon resonance).
 Basic cell biology techniques including western blotting, fluorescence activated cell
sorter, fluorescence and confocal microscopy.
 Bioinformatics programming tools such as Clustal X/ W, BLAST, PSI –BLAST, MEGA
etc.
 Molecular modelling and simulation software’s such as Modeller, I-TASSER, NACESS,
ABPS etc.
.
AWARDS AND HONORS
Vidyasagar College (Undergraduate College, India) – Proficiency Award for BSc
Examination, October 2004.
Qualified GATE-2007 examination with 95 percentile conducted by Ministry of Human
Resource Development (MHRD), India.
Institute Fellowship awarded by Bose Institute, India for pursuing PhD studentship.
Council of Scientific and Industrial Research (CSIR) Senior Research Fellowship (SRF)
awarded by CSIR, India.
Professor BB Biswas outstanding student of the year awarded by Bose Institute, India for
the year 2011.
Travel grant received from Department of Science and Technology (DST), India and
Council of Scientific and Industrial Research (CSIR), India to attend Colloid and
Nanomedicine 2012, at Amsterdam, Netherland.
Awarded 1 year postdoctoral fellowship from the Labex consortium (2013), France
Travel grant received by the conference organizer to attend “International Conference on
Bimolecular Forms and Functions” organized by Indian Institute of Science held at
Bangalore during January 8-11, 2013.
Research Work Synopsis
Post Doctoral:
Microtubules are essential in cell division, intracellular transport, as well as in cell differentiation (e.g. neurons) and organelle assembly (e.g. cilia)however in most organisms, microtubules are extensively heterogeneous due to expression of multiple tubulin genes as well as to posttranslational modifications of tubulin.Our present understanding of how the diversity of the tubulin molecules affects microtubule functions and thus cellular activity is very limited. One of the long-lasting difficulties in the field is the absence of experimental systems – cellular as well as biophysical – that would allow direct measurements of the impact of tubulin heterogeneity on microtubule properties and functions. In my present project, I am establishing fission yeast as a model system to address these questions. Fission yeast has a simple microtubule cytoskeleton, which carries none of the known posttranslational modifications and is assembled from only two alpha and one beta-tubulin gene product. This unique situation together with the possibility of genetically modifying the yeast genome by DNA recombination will be used to systematically introduce different tubulin isotypes, chimeric tubulins as well as controlled posttranslational modifications, and test their impact on microtubule behavior. Using this approach, I am particularly focusing on the roles of tubulin C-terminal tails, their heterogeneity and posttranslational regulation in the control of microtubule interactions within the yeast cytoskeleton. While the analysis of potential phenotypes in yeast will guide further cell-biological studies, I am in parallel purifying recombinant yeast tubulin for in vitro biophysical experiments. In order to adapt these experiments to the small amounts of tubulin that can be purified from yeast, I have already develop microfluidic systems as tools to perform single molecule
Pre Doctoral:

During my PhD, I was working in chemical biology, focusing on the design and functional
analysis ofprotein-nanoparticles interaction and I believe my research has provided significant insight on issue likeprotein nanoparticle recognition process.In brief, I have investigated the effect of ZnO nanoparticles on structure, stability and activity of diverse sets of protein (wild type and recombinant) such as lysozyme, a-lactalbumin and bovine serum albumin (BSA), ToxRp (a transcription factor related to pathogenicity in Vibrio cholerae). Using different biophysical and microscopic techniques the structural changes in proteins were elucidated, and the data were finally interpreted in terms of the three dimensional structure. The binding interaction between ZnO and proteins can be further classified into three types: i) electrostatic (with lysozyme), ii) hydrophobic (with alpha- lactalbumin and ToxRp) and iii) mixed (involving both hydrophobic and electrostatic with BSA). We further found that hydrophobic interactions are deleterious to protein structure function and activity compared to electrostatic interactions, which stabilizes the protein. Intuitively, I have conjugated an anti-malarial drug chloroquine with gold-nanoparticle to study the bio-distribution, and also investigated its pH dependent release and cytotoxicity towards target cells. Another extension of my investigation dealt with the effect ZnO NPs on different multi drug resistant bacteria cells (including Vibrio cholera and Helicobacter pylori) and to investigate its mode of action. Furthermore I have also studied the impact of drug molecules on protein structure function and dynamics. I have also determined tubulin as a molecular target of curcumin (a tradition Indian medicine). Taken together my scientific work has lead to more than 18 peer-reviewed publications and one patent.

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